Full Title CELL CULTURE PROCESSING

Short Title CELL CULTURE PROCESSING

Code BIO08045
Level 08
Credit 05

Author Butler, Mary
Department Life Sciences

Subject Area Bio Tech/Eng/Chem
Attendence N/A%
Fee

Description
This module aims to provide the student with a broad understanding of the theory of cell culture processing as it pertains to the biosynthesis of modern biopharmaceutical products. It will deal with the processing flow from cell vial thaw and subsequent scale-up through to protein synthesis and product capture.

Indicative Syllabus
1. Overview of Cell Biology relating to Cell Culture Processing and Bioreactor Operations.

2. Kinetics of Cell Growth, Yield and Productivities, cell counting techniques, cell density and cell viability measurements.

3. Cell Culture Media Design and Formulation.

4. Energy and Mass Transfer Systems for Bioprocessing.

5. Bioreactor Design and Engineering.

6. Bioreactor Operation and Control of critical parameters for protein synthesis.

Learning Outcomes
On completion of this module the learner will/should be able to
  1. Describe the fundamentals of cell biology, cell culture processing and explain the process kinetics relating to cell growth  and productivity for a bioreactor process.

  2. Explain the different types and formulations of media available and their overall importance in optimising the cell culture process. 

  3. Describe the various bioreactor designs available and their key features including relative advantages and disadvantages of different operating modes.

  4. Describe the main operating and control loops for a modern bioreactor and the critical control parameters associated with bioreactor performance. 

  5. Analyse, evaluate and draw conclusions from data obtained from lab. demonstrations and/or data obtained from problem solving exercises.

  6. Perform research on a relevant topic and analyse, evaluate and draw conclusions accordingly.

  7. Communicate scientific information and data in an appropriate format.


Assessment Strategies
Continuous Assessment of Course Work - 30% of marks.

End of term online exam - 30% of marks.

Mini research project - 40% of marks.

The miniproject is a MANDATORY form of assessment for any modules on this programme with a miniproject component.

Module Dependencies
Pre Requisite Modules
Fundermentals of cell biology
Co Requisite Modules
None
Incompatible Modules
None

Coursework Assessment Breakdown %
Course Work / Continuous Assessment 70 %
End of Semester / Year Formal Examination 30 %

Coursework Assessment Breakdown

Description Outcome Assessed % of Total Assessment Week
Open Book Exam Online 1,2 15 Week 4
Open Book Exam Online. 3,4 15 Week 9
Individual Project Research and Problem Solving 5,6,7 40 Week 15


End Exam Assessment Breakdown

Description Outcome Assessed % of Total Assessment Week
Other Exam Online. 1,2,3,4 30 Week 13


Distance Learning Mode Workload

Type Location Description Hours Frequency Avg Weekly Workload

Total Average Weekly Learner Workload 0.00 Hours

Part Time Mode Workload

Type Location Description Hours Frequency Avg Weekly Workload
Lecture Not Specified Online lecture 2 Weekly 2.00
Directed Learning Not Specified Course Notes. 4 Weekly 4.00
Independent Learning UNKNOWN Mini Research Activities. 4 Weekly 4.00

Total Average Weekly Learner Workload 6.00 Hours

Full Time Mode Workload

Type Location Description Hours Frequency Avg Weekly Workload

Total Average Weekly Learner Workload 0.00 Hours

Online Learning Mode Workload

Type Location Description Hours Frequency Avg Weekly Workload

Total Average Weekly Learner Workload 0.00 Hours

Resources
Book Resources
'Pharmaceutical Biotechnology - Concepts and Applications' by Gary Walsh.

Wiley Publications. 2007. ISBN - 978-0-470-01244-4.



'Basic Biotechnology' by Colin Ratledge and Bjorn Kristiansen.

Cambridge Press. 2001. ISBN - 0-521-779170.



'Bioprocess Engineering - Basic Concepts' by Shuler,M.L. and Kargi F.

Prentice-Hall. 2002. ISBN - 0-13-081908-5.
Other Resources
None
Url Resources
Additional Info