Full Title PROTEIN PURIFICATION

Short Title PROTEIN PURIFICATION

Code BIO08044
Level 08
Credit 05

Author Butler, Mary
Department Life Sciences

Subject Area Bio Tech/Eng/Chem
Attendence N/A%
Fee

Description
This course aims to provide the student with a broad understanding of the main theoretical concepts and principles of protein purification (downstream processing) from product capture at the end of the cell culture process through the various stages of protein separation and purification including viral elimination.

Indicative Syllabus
1. Introduction to Protein Purification.

2. The Design and Operation of an effective Protein Capture System.

3. The Theory and Principles of Chromatography for Purification.

4. The Different Types of Chromatography Systems for Purification.

5. Other Purification Technologies - Microfiltration, Ultrafiltration, Diafiltration, Centrifugation.

6. Viral Exclusion Technologies for Protein Purification. Viral contamination problems and viral filtration steps.

7. Quality control techniques for protein purification.

Learning Outcomes
On completion of this module the learner will/should be able to
  1. Outline the main elements of an effective and efficient downstream process design for protein capture and purification.

  2. Describe the general theory and main pirnciples of chromatography technology for protein purification applications including the different types of chromatography systems available.

  3. Explain other downstream processing technologies such as microfiltration, ultrafiltration, diafiltration,  centrifugation.

  4. Describe the various viral exclusion and elimination technologies for downstream processing as well as relevant quality control methods.

  5. Perform research on a relevant topic and analyse, evaluate and draw conclusions accordingly.

  6. Communicate scientific information and data in an appropriate format and manner.


Assessment Strategies
Continuous Assessment of Course Work - 30% of marks.

End of term online exam - 30% of marks.

Mini research project - 40% of marks.

The miniproject is a MANDATORY form of assessment for any modules on this programme with a miniproject component.

Module Dependencies
Pre Requisite Modules
None
Co Requisite Modules
None
Incompatible Modules
None

Coursework Assessment Breakdown %
Course Work / Continuous Assessment 70 %
End of Semester / Year Formal Examination 30 %

Coursework Assessment Breakdown

Description Outcome Assessed % of Total Assessment Week
Open Book Exam Online 1,2 15 Week 4
Open Book Exam Online 3,4 15 Week 9
Individual Project Mini Research 5,6 40 Week 15


End Exam Assessment Breakdown

Description Outcome Assessed % of Total Assessment Week
Other Exam Online 1,2,3,4 30 Week 13


Distance Learning Mode Workload

Type Location Description Hours Frequency Avg Weekly Workload

Total Average Weekly Learner Workload 0.00 Hours

Part Time Mode Workload

Type Location Description Hours Frequency Avg Weekly Workload
Lecture Not Specified Online 2 Weekly 2.00
Directed Learning Not Specified Course Notes 4 Weekly 4.00
Independent Learning UNKNOWN Mini Research Activities 4 Weekly 4.00

Total Average Weekly Learner Workload 6.00 Hours

Full Time Mode Workload

Type Location Description Hours Frequency Avg Weekly Workload

Total Average Weekly Learner Workload 0.00 Hours

Online Learning Mode Workload

Type Location Description Hours Frequency Avg Weekly Workload

Total Average Weekly Learner Workload 0.00 Hours

Resources
Book Resources
'Pharmaceutical Biotechnology - Concepts and Applications' by Gary Walsh.

Wiley Publications. 2007. ISBN - 978-0-470-01244-4.

'Bioseparations Science and Engineering' by R.G. Harrison, P.Todd, S.R. Rudge, D.P. Petrides. Oxford University Press. 2003. ISBN - 0-19-512340-9.

'Isolation and Purification of Proteins' by R. Hatti-Kaul, B.Mattiasson.

Marcel-Dekker. 2003. ISBN- 0-8247-0726-5.
Other Resources
None
Url Resources
Additional Info