Full Title Protein Purification L7.

Short Title Protein Purification L7.

Code BIO07011
Level 07
Credit 10

Author Tobin, Kieran
Department Applied Sciences

Subject Area Bio Tech/Eng/Chem
Attendence N/A%
Fee

Description
This course aims to provide students with a broad understanding and practical appreciation of the main theoretical concepts and principles of protein purification (downstream processing). This will include both theory some relevant laboratory practicals.

Indicative Syllabus
Introduction to Protein Purification.

The Design and Operation of an effective Protein Capture System.

The Theory and Principles of Chromatography for Purification.

The Theory and Principles of Filtration for Purification.

Practical chromatography and filtration applications for Purification.

Viral Exclusion Technologies for Protein Purification.


Learning Outcomes
On completion of this module the learner will/should be able to
  1. Outline the main elements of a downstream process design for protein capture and purification.

  2. Describe the general theory and main principles of chromatography technology for protein purification applications.

  3. Describe the general theroy and main principles of filtration technology for protein purification applications.

  4. Describe the various viral exclusion and elimination technologies for downstream processing.

  5. Perform a range of laboratory and pilot plant practicals relating to protein purification.

  6. Communicate scientific information and data in an appropriate format and manner.


Assessment Strategies
A combination of continuous assessment strategies, some laboratory experiments and an end-of-term exam.

Assessment Facilitites
Online teaching technologies.

Module Dependencies
Pre Requisite Modules
None
Co Requisite Modules
None
Incompatible Modules
None

Coursework Assessment Breakdown %
Course Work / Continuous Assessment 70 %
End of Semester / Year Formal Examination 30 %

Coursework Assessment Breakdown

Description Outcome Assessed % of Total Assessment Week
Continuous Assessment Short-form exams and lab. practical write-ups. 1,2,3,4,5,6 70 OnGoing


End Exam Assessment Breakdown

Description Outcome Assessed % of Total Assessment Week
Final Exam 1,2,3,4 30 End of Term


Distance Learning Mode Workload

Type Location Description Hours Frequency Avg Weekly Workload

Total Average Weekly Learner Workload 0.00 Hours

Part Time Mode Workload

Type Location Description Hours Frequency Avg Weekly Workload
Lecture Distance Learning Suite Online lecture. 1 Weekly 1.00
Laboratory Practical Science Laboratory Laboratory practical experiments. 16 Once Per Semester 1.07
Directed Learning Not Specified Directed self-study. 5 Weekly 5.00

Total Average Weekly Learner Workload 7.07 Hours

Full Time Mode Workload

Type Location Description Hours Frequency Avg Weekly Workload

Total Average Weekly Learner Workload 0.00 Hours

Online Learning Mode Workload

Type Location Description Hours Frequency Avg Weekly Workload

Total Average Weekly Learner Workload 0.00 Hours

Resources
Book Resources
'Pharmaceutical Biotechnology - Concepts and Applications' by Gary Walsh.

Wiley Publications. 2007. ISBN - 978-0-470-01244-4.

'Bioseparations Science and Engineering' by R.G. Harrison, P.Todd, S.R. Rudge, D.P. Petrides. Oxford University Press. 2003. ISBN - 0-19-512340-9.

'Isolation and Purification of Proteins' by R. Hatti-Kaul, B.Mattiasson.

Marcel-Dekker. 2003. ISBN- 0-8247-0726-5.
Other Resources
None
Url Resources
Additional Info